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‹ Tue · 5 May 2026
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Artificial intelligence-powered H&E-based quantification of spatial tumor-infiltrating lymphocyte distribution identifies prognostic immune niches in colorectal cancer

AI analysis of routine tissue slides identifies immune cell patterns that predict cancer recurrence as reliably as specialized staining techniques.

AI analysis of H&E slides in 202 stage II–III CRC patients shows that spatial TIL distribution at the tumor-stromal border (TSB score) is an independent prognostic marker for recurrence-free survival, matching Immunoscore performance and working without additional IHC staining. AI-human agreement was comparable to inter-pathologist reliability, supporting potential clinical integration of AI-based spatial immune profiling from routine H&E slides.

What the study was

Study design
Retrospective cohort study with AI-based image analysis and validation against Immunoscore
Population
Stage II–III CRC patients (n=202), South Korea
Sample size
202
Category
Diagnostics
Maturity
Validated
Journal
Cancer Immunology, Immunotherapy

Why it surfaced

Validated AI-based spatial immune profiling from routine H&E achieving Immunoscore-equivalent prognosis; reduces cost and complexity of immune profiling in CRC.

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