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‹ Thu · 14 May 2026
Promising but preliminary

MUC4-targeted CAR-T Cells Restrain Chemotherapy-resistant Colorectal Cancer

Lab-engineered immune cells attack chemotherapy-resistant colon cancer in mice through a protein called MUC4, suggesting a new route for patients whose tumors resist standard drugs.

This preprint reports the engineering and preclinical evaluation of MUC4-targeted CAR-T cells against chemotherapy-resistant colorectal cancer, demonstrating efficient tumor elimination in vitro and improved survival in an aggressive intraperitoneal CRC metastasis model in NSG mice with no off-target toxicity. MUC4 is proposed as a broadly expressed surface antigen on mucinous, chemoresistant CRC subtypes where standard therapy fails.

What the study was

Study design
Preclinical — in vitro + xenograft mouse model
Population
MUC4-expressing CRC cell lines (HT29-MTX, T84); NSG mouse intraperitoneal model
Category
Treatment Innovation
Maturity
Exploratory
Journal
bioRxiv

Why it surfaced

Novel CAR-T target for chemoresistant CRC with preclinical efficacy data; preprint + non-human model caps at 5. PMC full text available (PMC13160141).

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