Laboratory Concordance and Prognostic Impact of 10-Color Flow Cytometry and Next-Generation Sequencing-Based Immunoglobulin Gene Assay for Measurable Residual Disease Monitoring in Adult B-Cell Acute Lymphoblastic Leukemia: A Multicenter Study From Thai Acute Leukemia Working Group (TALWG).
Flow cytometry offers a practical way to detect residual leukemia cells and predict relapse risk in resource-limited settings.
This prospective multicenter study from the Thai Acute Leukemia Working Group enrolled 51 newly diagnosed adult B-ALL patients and compared 10-color flow cytometry with NGS-based immunoglobulin heavy chain clonality assays for measurable residual disease monitoring at three timepoints. MRD negativity at 3 months by either method predicted superior relapse-free survival, with flow cytometry independently predicting relapse, establishing FC as a practical MRD platform for resource-limited clinical settings.
What the study was
- Study design
- prospective multicenter cohort study
- Category
- Genomics/Precision Medicine
- Maturity
- Validated
- Journal
- International Journal of Laboratory Hematology
Why it surfaced
Prospective multicenter validation of FC vs. NGS MRD assays in B-ALL with prognostic evidence; directly addresses T1 hematologic malignancy watchlist and clinical translation in resource-constrained healthcare systems.
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